抗繆勒管激素(AMH)檢測試劑盒(化學發光免疫分析法)

CLIA Kit for Anti-Mullerian Hormone (AMH)

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance

  • 抗繆勒管激素(AMH)檢測試劑盒(化學發光免疫分析法) 產品包裝(模擬)
  • 抗繆勒管激素(AMH)檢測試劑盒(化學發光免疫分析法) 產品包裝(模擬)
  • 抗繆勒管激素(AMH)檢測試劑盒(化學發光免疫分析法) 實驗結果圖
  • CCA228Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

天天酷跑未来少年穿越日 www.mncds.icu 本試劑盒用于檢測抗繆勒管激素(AMH),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的抗繆勒管激素(AMH)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-105 97
EDTA plasma(n=5) 84-98 90
heparin plasma(n=5) 91-105 102

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的抗繆勒管激素(AMH),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中抗繆勒管激素(AMH)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 87-104% 78-97% 85-92%
EDTA plasma(n=5) 84-99% 96-105% 87-94% 98-105%
heparin plasma(n=5) 87-94% 80-96% 85-104% 92-101%

穩定性

經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)50µL,
    加入50µL檢測液A(臨用前配制);
    37°C溫育1小時。
3. 洗板3次;
4. 加檢測溶液B100µL,37°C孵育30分鐘;
5. 洗板5次;
6. 加底物100µL,37°C孵育10分鐘;
7. 讀數。

實驗原理

本試劑盒應用競爭抑制酶聯免疫分析法測定標本中待測物質水平。將抗繆勒管激素(AMH)單克隆抗體包被微孔板,制成固相載體,往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本),待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經洗滌去掉未結合物,然后加入HRP標記的親和素,再次徹底洗滌后加入底物。加入底物后會產生輝光型光信號。發射光強度和樣品中的抗繆勒管激素(AMH)呈負相關。用化學發光儀測定相對光單位(RLU),計算樣品濃度。

相關產品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPA228Mu01 抗繆勒管激素(AMH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA228Mu01 抗繆勒管激素(AMH)多克隆抗體 WB; IHC; ICC; IP.
CEA228Mu 抗繆勒管激素(AMH)檢測試劑盒(酶聯免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA228Mu 抗繆勒管激素(AMH)檢測試劑盒(化學發光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA228Mu11 抗繆勒管激素(AMH)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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